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β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar

journal contribution
submitted on 2024-07-04, 13:04 and posted on 2024-07-04, 13:05 authored by Mazen A. Sid Ahmed, Faisal Ahmad Khan, Ali A. Sultan, Bo Söderquist, Emad Bashir Ibrahim, Jana Jass, Ali S. Omrani

Background

The distribution of β-lactam resistance genes in P. aeruginosa is often closely related to the distribution of certain high-risk international clones. We used whole-genome sequencing (WGS) to identify the predominant sequence types (ST) and β-lactamase genes in clinical isolates of multidrug-resistant (MDR)-P. aeruginosa from Qatar

Methods

Microbiological identification and susceptibility tests were performed by automated BD Phoenix™ system and manual Liofilchem MIC Test Strips.

Results

Among 75 MDR-P. aeruginosa isolates; the largest proportions of susceptibility were to ceftazidime-avibactam (n = 36, 48%), followed by ceftolozane-tazobactam (30, 40%), ceftazidime (n = 21, 28%) and aztreonam (n = 16, 21.3%). All isolates possessed Class C and/or Class D β-lactamases (n = 72, 96% each), while metallo-β-lactamases were detected in 20 (26.7%) isolates. Eight (40%) metallo-β-lactamase producers were susceptible to aztreonam and did not produce any concomitant extended-spectrum β-lactamases. High risk ST235 (n = 16, 21.3%), ST357 (n = 8, 10.7%), ST389 and ST1284 (6, 8% each) were most frequent. Nearly all ST235 isolates (15/16; 93.8%) were resistant to all tested β-lactams.

Conclusion

MDR-P. aeruginosa isolates from Qatar are highly resistant to antipseudomonal β-lactams. High-risk STs are predominant in Qatar and their associated MDR phenotypes are a cause for considerable concern.

Other Information

Published in: Antimicrobial Resistance & Infection Control
License: http://creativecommons.org/licenses/by/4.0/
See article on publisher's website: https://dx.doi.org/10.1186/s13756-020-00838-y

Funding

Hamad Medical Corporation (IRGC-01–51-033).

Qatar National Research Fund (NPRP12S-0219-190109), Molecular and genomic epidemiology of invasive, multi drug resistant gram-negative bacteria in the State of Qatar.

Svenska Forskningsrådet Formas (219-2014-837).

History

Language

  • English

Publisher

Springer Nature

Publication Year

  • 2020

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License.

Institution affiliated with

  • Hamad Medical Corporation
  • Hamad General Hospital - HMC
  • Weill Cornell Medicine - Qatar

Methodology

Microbiological identification and susceptibility tests were performed by automated BD Phoenix™ system and manual Liofilchem MIC Test Strips.

Geographic coverage

Qatar

Usage metrics

    Hamad General Hospital - HMC

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