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Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies

journal contribution
submitted on 2024-05-08, 09:21 and posted on 2024-05-08, 09:21 authored by Hadi M. Yassine, Hadeel Al-Jighefee, Duaa W. Al-Sadeq, Soha R. Dargham, Salma N. Younes, Farah Shurrab, Reham M. Marei, Ali Ait. Hssain, Sara Taleb, Hashim Alhussain, Maryam A. Al-Nesf, Abdullatif Al-Khal, Hamda Qotba, Asmaa A. Althani, Patrick Tang, Laith J. Abu-Raddad, Gheyath K. Nasrallah


To evaluate and compare the performances of five commercial ELISA assays (EDI, AnshLabs, Dia.Pro, NovaTec, and Lionex) for detecting anti-SARS-CoV-2 IgG.


Seventy negative control samples (collected before the COVID-19 pandemic) and samples from 101 RT-PCR-confirmed SARS-CoV-2 patients (collected at different time points from symptom onset: ≤7, 8–14 and >14 days) were used to compare the sensitivity, specificity, agreement, and positive and negative predictive values of each assay with RT-PCR. A concordance assessment between the five assays was also conducted. Cross-reactivity with other HCoV, non-HCoV respiratory viruses, non-respiratory viruses, and nuclear antigens was investigated.


Lionex showed the highest specificity (98.6%; 95% CI 92.3–99.8), followed by EDI and Dia.Pro (97.1%; 95% CI 90.2–99.2), NovaTec (85.7%; 95% CI 75.7–92.1), then AnshLabs (75.7%; 95% CI 64.5–84.2). All ELISA kits cross-reacted with one anti-MERS IgG-positive sample, except Lionex. The sensitivity was low during the early stages of the disease but improved over time. After 14 days from symptom onset, Lionex and NovaTec showed the highest sensitivity at 87.9% (95% CI 72.7–95.2) and 86.4% (95% CI 78.5–91.7), respectively. The agreement with RT-PCR results based on Cohen’s kappa was as follows: Lionex (0.89) > NovaTec (0.70) > Dia.Pro (0.69) > AnshLabs (0.63) > EDI (0.55).


The Lionex and NovaLisa IgG ELISA kits, demonstrated the best overall performance.

Other Information

Published in: International Journal of Infectious Diseases
See article on publisher's website:

Additional institutions affiliated with: Sidra Medicine



  • English



Publication Year

  • 2021

License statement

This Item is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Institution affiliated with

  • Qatar University
  • Biomedical Research Center - QU
  • Qatar University Health - QU
  • College of Health Sciences - QU HEALTH
  • College of Medicine - QU HEALTH
  • Weill Cornell Medicine - Qatar
  • Hamad Medical Corporation
  • Hamad Bin Khalifa University
  • College of Health and Life Sciences - HBKU
  • Primary Health Care Corporation

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