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The Cancer Testis Antigen PRAME: Expression in Triple Negative Breast Cancer

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submitted on 2025-03-17, 09:30 and posted on 2025-03-17, 09:30 authored by Ghaneya M. Al-Khadairi

Background

Triple Negative Breast Cancer (TNBC) is a subtype of breast cancer that is clinically characterized by the absence of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2). TNBC is a heterogeneous disease with distinct molecular profile, aggressive nature, poor prognosis and lack of targeted therapies. Recent studies demonstrate that TNBC may be a cancer testis antigen (CTA)- rich tumor, raising the possibility of CTA-based immunotherapy and cancer vaccines as a potential therapeutic approach for patients with TNBC. The focus of this study was to investigate the expression of preferentially expressed antigen of melanoma (PRAME), one member of the CTA family, in TNBC and non-TNBC cell lines in order to select appropriate cell lines to enable future research into functional role of PRAME in the progression of TNBC.

Methods

Five TNBC cell lines (MDA-MB-231, MDA-MB-468, MDA-MB-453, HCC70 and BT549) along with one non-TNBC cell line (MCF7) were used to investigate the expression of PRAME using qPCR, western blot and immunofluorescence techniques.

Results

PRAME expression was detected in 2 out of 5 TNBC cell lines (HCC70 and BT549), both represents different molecular TNBC subtypes. Higher expression of PRAME was detected in HCC70 compared to BT549.

Conclusion

At the end, our study showed that PRAME expression isn't specific to TNBC tumors overall or to TNBC molecular subtypes.

History

Language

  • English

Publication Year

  • 2017

License statement

© The author. The author has granted HBKU and Qatar Foundation a non-exclusive, worldwide, perpetual, irrevocable, royalty-free license to reproduce, display and distribute the manuscript in whole or in part in any form to be posted in digital or print format and made available to the public at no charge. Unless otherwise specified in the copyright statement or the metadata, all rights are reserved by the copyright holder. For permission to reuse content, please contact the author.

Institution affiliated with

  • Hamad Bin Khalifa University
  • College of Science and Engineering - HBKU

Degree Date

  • 2017

Degree Type

  • Master's

Advisors

Julie Decock

Committee Members

Ahmad Abushaikha ; Mohammed Emara ; Imed Gallouzi ; Konstantinos Verkrellis

Department/Program

College of Science and Engineering

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