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Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers

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submitted on 2023-06-08, 05:37 and posted on 2023-06-13, 10:50 authored by Houda Yasmine Ali Moussa, Nimshitha Manaph, Gowher Al, Selma Maacha, Kyung Chul Shin, Samia M. Ltaief, Vijay Gupta, Yongfeng Tong, Janarthanan Ponraj, Salam Salloum-Asfa, Said Mansour, Fouad A. Al-Shaban, Hyung-Goo Kim, Lawrence W. Stanton, Jean-Charles Grivel, Sara A. Abdulla, Abeer R. Al-Shammari, Yongsoo Park

Extracellular vesicles (EVs) are membrane vesicles released from cells to the extracellular space, involved in cell-to-cell communication by the horizontal transfer of biomolecules such as proteins and RNA. Because EVs can cross the blood-brain barrier (BBB), circulating through the bloodstream and reflecting the cell of origin in terms of disease prognosis and severity, the contents of plasma EVs provide non-invasive biomarkers for neurological disorders. However, neuronal EV markers in blood plasma remain unclear. EVs are very heterogeneous in size and contents, thus bulk analyses of heterogeneous plasma EVs using Western blot and ELISA have limited utility. In this study, using flow cytometry to analyze individual neuronal EVs, we show that our plasma EVs isolated by size exclusion chromatography are mainly CD63-positive exosomes of endosomal origin. As a neuronal EV marker, neural cell adhesion molecule (NCAM) is highly enriched in EVs released from induced pluripotent stem cells (iPSCs)-derived cortical neurons and brain organoids. We identified the subpopulations of plasma EVs that contain NCAM using flow cytometry-based individual EV analysis. Our results suggest that plasma NCAM-positive neuronal EVs can be used to discover biomarkers for neurological disorders. 

Other Information

Published in: Frontiers in Integrative Neuroscience
License: https://creativecommons.org/licenses/by/4.0/
See article on publisher's website: http://dx.doi.org/10.3389/fnint.2022.879832 

Funding

Open Access funding provided by the Qatar National Library.

History

Language

  • English

Publisher

Frontiers

Publication Year

  • 2022

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License.

Institution affiliated with

  • Hamad Bin Khalifa University
  • Qatar Biomedical Research Institute - HBKU
  • Neurological Disorders Research Center - QBRI
  • Qatar Environment and Energy Research Institute - HBKU
  • College of Health and Life Sciences - HBKU
  • Sidra Medicine

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