Manara - Qatar Research Repository
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Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells

journal contribution
submitted on 2024-05-20, 11:41 and posted on 2024-05-20, 11:43 authored by Manale Karam, Ihab Younis, Noor R. Elareer, Sara Nasser, Essam M. Abdelalim

Human pluripotent stem cells (hPSCs) can provide unlimited supply for mesenchymal stem cells (MSCs) and adipocytes that can be used for therapeutic applications. Here we developed a simple and highly efficient all-trans-retinoic acid (RA)-based method for generating an off-the-shelf and scalable number of human pluripotent stem cell (hPSC)-derived MSCs with enhanced adipogenic potential. We showed that short exposure of multiple hPSC lines (hESCs/hiPSCs) to 10 μM RA dramatically enhances embryoid body (EB) formation through regulation of genes activating signaling pathways associated with cell proliferation, survival and adhesion, among others. Disruption of cell adhesion induced the subsequent differentiation of the highly expanded RA-derived EB-forming cells into a pure population of multipotent MSCs (up to 1542-fold increase in comparison to RA-untreated counterparts). Interestingly, the RA-derived MSCs displayed enhanced differentiation potential into adipocytes. Thus, these findings present a novel RA-based approach for providing an unlimited source of MSCs and adipocytes that can be used for regenerative medicine, drug screening and disease modeling applications.

Other Information

Published in: Cells
See article on publisher's website:


Studying insulin resistance in a Qatari cohort using patient-specific induced pluripotent stem -cells



  • English



Publication Year

  • 2020

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License.

Institution affiliated with

  • Hamad Bin Khalifa University
  • Qatar Biomedical Research Institute - HBKU
  • Diabetes Research Center - QBRI
  • College of Health and Life Sciences - HBKU
  • Carnegie Mellon University in Qatar