Manara - Qatar Research Repository
10.1002_dta.3157.pdf (953.13 kB)

Red blood cell derived extracellular vesicles during the process of autologous blood doping

Download (953.13 kB)
journal contribution
submitted on 2023-03-14, 15:34 and posted on 2023-03-14, 15:40 authored by S. C. Voss, M. Yassin, J. C. Grivel, S. Al Hmissi, N. Allahverdi, A. Nashwan, Z. Merenkov, M. Abdulla, A. Al Malki, C. Raynaud, W. Elsaftawy, A. Al Kaabi, F. Donati, F. Botre, V. Mohamed Ali, C. Georgakopoulos, M. Al Maadheed

The purpose of this pilot study was to investigate the effects of the transfusion of one erythrocyte concentrate on the number of circulating red blood cell extracellular vesicles (RBC-EVs) and their clearance time. Six, healthy volunteers donated their blood and were transfused with their RBC concentrate after 35–36 days of storage. One K2EDTA and one serum sample were collected before donation, at four timepoints after donation and at another six timepoints after transfusion. RBC-EVs were analyzed on a Cytek Aurora flow cytometer. A highly significant increase (p < 0.001) of RBC-EVs from an average of 60.1 ± 19.8 (103/μL) at baseline to 179.3 ± 84.7 (103/μL) in the first 1–3 h after transfusion could be observed. Individual differences in the response to transfusion became apparent with one volunteer showing no increase and another an increased concentration at one timepoint after donation due to an influenza infection. We concluded that in an individualized passport approach, increased RBC-EVs might be considered as additional evidence when interpreting suspicious Athletes Biological Passport (ABPs) but for this additional research related to sample collection and transport processes as well as method development and harmonization would be necessary. 

Other Information

Published in: Drug Testing and Analysis
See article on publisher's website:



  • English



Publication Year

  • 2021

Institution affiliated with

  • Anti-Doping Laboratory Qatar
  • Hamad Medical Corporation
  • Sidra Medical and Research Center

Usage metrics

    Manara - Qatar Research Repository


    Ref. manager