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RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis

journal contribution
submitted on 2024-06-04, 11:11 and posted on 2024-06-11, 11:14 authored by Reem Saleh, Varun Sasidharan Nair, Mahmood Al-Dhaheri, Mahwish Khawar, Mohamed Abu Nada, Nehad M. Alajez, Eyad Elkord

Elevated levels of myeloid-derived suppressor cells (MDSCs), including polymorphonuclear MDSCs (PMN-MDSCs) and immature MDSCs (I-MDSCs), are usually associated with disease progression in cancer patients, including colorectal cancer (CRC). However, biological mechanisms and molecular pathways regulated by MDSC subpopulations in the CRC tumor microenvironment (TME) have not been fully investigated. In this study, we performed transcriptomic analysis of tumor-infiltrating I-MDSCs and PMN-MDSCs isolated from tumor tissues of six CRC patients, compared to antigen-presenting cells (APCs). We also compared the transcriptomic profiles of tumor-infiltrating PMN-MDSCs to I-MDSCs. Our results showed different molecular pathways regulated by each MDSC subset, potentially reflecting their phenotypical/molecular/functional characteristics in the CRC TME. Moreover, we identified gene signatures in PMN-MDSC and I-MDSC of poor overall survival (OS) and disease-free survival (DFS) using the Cancer Genome Atlas (TCGA) dataset from patients with colon adenocarcinoma (COAD). However, functional studies are required to validate these findings.

Other Information

Published in: Frontiers in Oncology
License: https://creativecommons.org/licenses/by/4.0/
See article on publisher's website: https://dx.doi.org/10.3389/fonc.2020.604906

Funding

Qatar Biomedical Research Institute, Hamad Bin Khalifa University, Qatar Foundation (VR04).

History

Language

  • English

Publisher

Frontiers

Publication Year

  • 2020

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License.

Institution affiliated with

  • Hamad Bin Khalifa University
  • Qatar Biomedical Research Institute - HBKU
  • Cancer Research Center - QBRI
  • Hamad Medical Corporation
  • Hamad General Hospital - HMC

Related Datasets

Saleh, R., Sasidharan Nair, V., Al-Dhaheri, M., Khawar, M., Abu Nada, M., Alajez, N. M., & Elkord, E. (2020). RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis. Frontiers in Oncology, 10. https://doi.org/10.3389/fonc.2020.604906. National Library of Medicine : National Center for Biotechnology Information : BioProject ID PRJNA664006. Last modified 2020. https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA664006.