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10.1016_j.heliyon.2023.e15560.pdf (10.03 MB)

Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles

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submitted on 2024-01-25, 06:47 and posted on 2024-01-25, 06:48 authored by Mohamed Qasim Al-Fahdawi, Ahmed Faris Aldoghachi, Fatah H. Alhassan, Faris A.J. Al-Doghachi, Hussah Abdullah Alshwyeh, Abdullah Rasedee, Sulaiman Mohammed Alnasser, Mothanna Sadiq Al-Qubaisi, Wisam Nabeel Ibrahim

Silver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurements, and dispersive X-ray spectroscopy (EDX). The morphology of Ag/MgO nanoparticles was determined by transmission and scanning electron microscopy, which revealed cuboidal shaped nanoparticles with sizes ranging from 31 to 68 nm and an average size of 43.5 ± 10.6 nm. The anticancer effects of Ag/MgO nanoparticles were evaluated on human colorectal (HT29) and lung adenocarcinoma (A549) cell lines, and their caspase-3, -8, and -9 activities, as well as Bcl-2, Bax, p53, cytochrome C protein expressions were estimated. Ag/MgO nanoparticles showed selective toxicity towards HT29 and A549 cells while remaining relatively innocuous towards the normal human colorectal, CCD-18Co, and lung, MRC-5 cells. The IC50 values of Ag/MgO nanoparticles on the HT29 and A549 cells were found to be 90.2 ± 2.6 and 85.0 ± 3.5 μg/mL, respectively. The Ag/MgO nanoparticles upregulated caspase-3 and -9 activities, downregulated Bcl-2, upregulated Bax and p53 protein expressions in the cancer cells. The morphology of the Ag/MgO nanoparticle treated HT29 and A549 cells was typical of apoptosis, with cell detachment, shrinkage, and membrane blebbing. The results suggest that Ag/MgO nanoparticles induce apoptosis in cancer cells and exhibit potential as a promising anticancer agent.

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Published in: Heliyon
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Publication Year

  • 2023

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  • Qatar University
  • Qatar University Health - QU
  • College of Health Sciences - QU HEALTH

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