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Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies

Version 2 2024-04-02, 07:00
Version 1 2022-11-22, 21:17
journal contribution
revised on 2024-04-02, 06:50 and posted on 2024-04-02, 07:00 authored by Yelda Birinci, Julia Preobraschenski, Marcelo Ganzella, Reinhard Jahn, Yongsoo Park

Large dense-core vesicles (LDCVs) contain a variety of neurotransmitters, proteins, and hormones such as biogenic amines and peptides, together with microRNAs (miRNAs). Isolation of LDCVs is essential for functional studies including vesicle fusion, vesicle acidification, monoamine transport, and the miRNAs stored in LDCVs. Although several methods were reported for purifying LDCVs, the final fractions are significantly contaminated by other organelles, compromising biochemical characterization. Here we isolated LDCVs (chromaffin granules) with high yield and purity from bovine adrenal medulla. The fractionation protocol combines differential and continuous sucrose gradient centrifugation, allowing for reducing major contaminants such as mitochondria. Purified LDCVs show robust acidification by the endogenous V-ATPase and undergo SNARE-mediated fusion with artificial membranes. Interestingly, LDCVs contain specific miRNAs such as miR-375 and miR-375 is stabilized by protein complex against RNase A. This protocol can be useful in research on the biological functions of LDCVs.

Author Correction: Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies: Scientific Reports, published online 06 October 2022.

Other Information

Published in: Scientific Reports
See article on publisher's website:



  • English


Springer Nature

Publication Year

  • 2020

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License.

Institution affiliated with

  • Hamad Bin Khalifa University
  • Qatar Biomedical Research Institute - HBKU
  • Neurological Disorders Research Center - QBRI