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Inter-Versus Intra-Host Sequence Diversity of pH1N1 and Associated Clinical Outcomes

journal contribution
submitted on 2024-07-11, 08:29 and posted on 2024-07-14, 13:28 authored by Hebah A. Al Khatib, Muna A. Al Maslamani, Peter V. Coyle, I. Richard Thompson, Elmoubasher A. Farag, Asmaa A. Al Thani, Hadi M. Yassine

The diversity of RNA viruses dictates their evolution in a particular host, community or environment. Here, we reported within- and between-host pH1N1virus diversity at consensus and sub-consensus levels over a three-year period (2015–2017) and its implications on disease severity. A total of 90 nasal samples positive for the pH1N1 virus were deep-sequenced and analyzed to detect low-frequency variants (LFVs) and haplotypes. Parallel evolution of LFVs was seen in the hemagglutinin (HA) gene across three scales: among patients (33%), across years (22%), and at global scale. Remarkably, investigating the emergence of LFVs at the consensus level demonstrated that within-host virus evolution recapitulates evolutionary dynamics seen at the global scale. Analysis of virus diversity at the HA haplotype level revealed the clustering of low-frequency haplotypes from early 2015 with dominant strains of 2016, indicating rapid haplotype evolution. Haplotype sharing was also noticed in all years, strongly suggesting haplotype transmission among patients infected during a specific influenza season. Finally, more than half of patients with severe symptoms harbored a larger number of haplotypes, mostly in patients under the age of five. Therefore, patient age, haplotype diversity, and the presence of certain LFVs should be considered when interpreting illness severity. In addition to its importance in understanding virus evolution, sub-consensus virus diversity together with whole genome sequencing is essential to explain variabilities in clinical outcomes that cannot be explained by either analysis alone.

Other Information

Published in: Microorganisms
License: https://creativecommons.org/licenses/by/4.0/
See article on publisher's website: https://dx.doi.org/10.3390/microorganisms8010133

Funding

Qatar University (QUCG-BRC-2018/2019-1).

Hamad Medical Corporation (16335/16).

History

Language

  • English

Publisher

MDPI

Publication Year

  • 2020

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License.

Institution affiliated with

  • Hamad Bin Khalifa University
  • Qatar Biomedical Research Institute - HBKU
  • College of Science and Engineering - HBKU
  • Hamad Medical Corporation
  • Communicable Disease Center - HMC
  • Ministry of Public Health
  • Qatar University
  • Biomedical Research Center - QU