Identification of distinct circulating microRNAs in acute ischemic stroke patients with type 2 diabetes mellitus
Stroke is the second leading cause of global mortality and continued efforts aim to identify predictive, diagnostic, or prognostic biomarkers to reduce the disease burden. Circulating microRNAs (miRNAs) have emerged as potential biomarkers in stroke. We performed comprehensive circulating miRNA profiling of ischemic stroke patients with or without type 2 diabetes mellitus (T2DM), an important risk factor associated with worse clinical outcomes in stroke. Serum samples were collected within 24 h of acute stroke diagnosis and circulating miRNAs profiled using RNA-Seq were compared between stroke patients with T2DM (SWDM; n = 92) and those without T2DM (SWoDM; n = 98). Our analysis workflow involved random allocation of study cohorts into discovery (n = 96) and validation (n = 94) datasets. Five miRNAs were found to be differentially regulated in SWDM compared to SWoDM patients. Hsa-miR-361-3p and -664a-5p were downregulated, whereas miR-423-3p, -140-5p, and -17-3p were upregulated. We also explored the gene targets of these miRNAs and investigated the downstream pathways associated with them to decipher the potential pathways impacted in stroke with diabetes as comorbidity. Overall, our novel findings provide important insights into the differentially regulated miRNAs, their associated pathways and potential utilization for clinical benefits in ischemic stroke patients with diabetes.
Other Information
Published in: Frontiers in Cardiovascular Medicine
License: https://creativecommons.org/licenses/by/4.0/
See article on publisher's website: https://dx.doi.org/10.3389/fcvm.2022.1024790
Funding
Qatar National Research Fund (NPRP11C-0115-180010), Qatar Diabetes Prevention Program (QDPP).
Hamad Medical Corporation, Qatar (15304/15).
History
Language
- English
Publisher
FrontiersPublication Year
- 2022
License statement
This Item is licensed under the Creative Commons Attribution 4.0 International License.Institution affiliated with
- Hamad Bin Khalifa University
- College of Health and Life Sciences - HBKU
- Qatar Biomedical Research Institute - HBKU
- Cancer Research Center - QBRI
- Diabetes Research Center - QBRI
- Neurological Disorders Research Center - QBRI
- Hamad Medical Corporation
- Neuroscience Institute - HMC
- Qatar Genome Program (2015-2024)