Manara - Qatar Research Repository
Browse
aljaber-et-al-2022-downregulation-of-cyp17a1-by-20-hydroxyecdysone-plasma-progesterone-and-its-vasodilatory-properties.pdf (2.08 MB)

Downregulation of CYP17A1 by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties

Download (2.08 MB)
journal contribution
submitted on 2024-04-01, 06:41 and posted on 2024-04-01, 06:42 authored by Maneera Y Aljaber, Nelson N Orie, Asmaa Raees, Suhail Kraiem, Mashael Al-Jaber, Waseem Samsam, Mostafa M Hamza, David Abraham, Norman M Kneteman, Alka Beotra, Vidya Mohamed-Ali, Mohammed Almaadheed

Aim: To investigate the effect of 20-hydroxyecdysone on steroidogenic pathway genes and plasma progesterone, and its potential impact on vascular functions. Methods: Chimeric mice with humanized liver were treated with 20-hydroxyecdysone for 3 days, and hepatic steroidogenic pathway genes and plasma progesterone were measured by transcriptomics and GC–MS/MS, respectively. Direct effects on muscle and mesenteric arterioles were assessed by myography. Results: CYP17A1 was downregulated in 20-hydroxyecdysone-treated mice compared with untreated group (p = 0.04), with an insignificant increase in plasma progesterone. Progesterone caused vasorelaxation which was blocked by 60 mM KCl, but unaffected by nitric oxide synthase inhibition. Conclusion: In the short term, 20-hydroxyecdysone mediates CYP17A1 downregulation without a significant increase in plasma progesterone, which has a vasodilatory effect involving inhibition of voltage-dependent calcium channels, and the potential to enhance 20-hydroxyecdysone vasorelaxation.


Other Information

Published in: Future Science OA
License: https://creativecommons.org/licenses/by/4.0/
See article on publisher's website: https://dx.doi.org/10.2144/fsoa-2022-0006

History

Language

  • English

Publisher

Future Science

Publication Year

  • 2022

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License

Institution affiliated with

  • Hamad Bin Khalifa University
  • Qatar Computing Research Institute - HBKU
  • Anti-Doping Laboratory Qatar - QA-DC