Manara - Qatar Research Repository
Browse
10.1016_j.xpro.2022.101613.pdf (4.26 MB)

Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells

Download (4.26 MB)
journal contribution
submitted on 2024-04-04, 08:13 and posted on 2024-04-04, 08:14 authored by Gowher Ali, Essam M. Abdelalim

The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).

Other Information

Published in: STAR Protocols
License: http://creativecommons.org/licenses/by/4.0/
See article on publisher's website: https://dx.doi.org/10.1016/j.xpro.2022.101613

History

Language

  • English

Publisher

Elsevier

Publication Year

  • 2022

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License.

Institution affiliated with

  • Hamad Bin Khalifa University
  • Qatar Biomedical Research Institute - HBKU
  • Diabetes Research Center - QBRI
  • College of Health and Life Sciences - HBKU

Usage metrics

    Qatar Biomedical Research Institute - HBKU

    Categories

    Keywords

    In vitro modelsHuman pluripotent stem cells (hPSCs)Retinoic acidSkin-related diseasesCell cultureCell therapy

    Licence

    CC BY 4.0

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC