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Differences in platelet‐rich plasma composition influence bone healing

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submitted on 2023-03-15, 08:05 and posted on 2023-03-15, 10:40 authored by Faez Saleh Al‐Hamed, Lina Abu‐Nada, Rania Rodan, Stylianos Sarrigiannidis, Jose Luis Ramirez‐Garcialuna, Hanan Moussa, Osama Elkashty, Qiman Gao, Tayebeh Basiri, Laura Baca, Jesus Torres, Lisa Rancan, Simon D. Tran, Marie Lordkipanidzé, Mari Kaartinen, Zahi Badran, Faleh Tamimi

Aim

Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing.

Materials and Methods

L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology.

Results

Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm3) compared to single-spin L-PRP (1.42 ± 0.27 mm3) or filtered L-PRP (1.38 ± 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP.

Conclusion

The preparation method of L-PRP affects their composition and potential to regenerate bone.

Other Information

Published in: Journal of Clinical Periodontology
License: http://creativecommons.org/licenses/by-nc/4.0/
See article on publisher's website: http://dx.doi.org/10.1111/jcpe.13546

History

Language

  • English

Publisher

Wiley

Publication Year

  • 2021

Institution affiliated with

  • Qatar University

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