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Correction: Discrimination of breast cancer from benign tumours using Raman spectroscopy

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submitted on 2024-07-10, 04:41 and posted on 2024-07-10, 04:43 authored by Fiona M. Lyng, Damien Traynor, Thi Nguyet Que Nguyen, Aidan D. Meade, Fazle Rakib, Rafif Al-Saady, Erik Goormaghtigh, Khalid Al-Saad, Mohamed H. Ali

Correction to: Discrimination of breast cancer from benign tumours using Raman spectroscopy., published online 14 February 2019.

Breast cancer is the most common cancer among women worldwide, with an estimated 1.7 million cases and 522,000 deaths in 2012. Breast cancer is diagnosed by histopathological examination of breast biopsy material but this is subjective and relies on morphological changes in the tissue. Raman spectroscopy uses incident radiation to induce vibrations in the molecules of a sample and the scattered radiation can be used to characterise the sample. This technique is rapid and non-destructive and is sensitive to subtle biochemical changes occurring at the molecular level. This allows spectral variations corresponding to disease onset to be detected. The aim of this work was to use Raman spectroscopy to discriminate between benign lesions (fibrocystic, fibroadenoma, intraductal papilloma) and cancer (invasive ductal carcinoma and lobular carcinoma) using formalin fixed paraffin preserved (FFPP) tissue. Haematoxylin and Eosin stained sections from the patient biopsies were marked by a pathologist. Raman maps were recorded from parallel unstained tissue sections. Immunohistochemical staining for estrogen receptor (ER) and human epidermal growth factor receptor 2 (HER2/neu) was performed on a further set of parallel sections. Both benign and cancer cases were positive for ER while only the cancer cases were positive for HER2. Significant spectral differences were observed between the benign and cancer cases and the benign cases could be differentiated from the cancer cases with good sensitivity and specificity. This study has shown the potential of Raman spectroscopy as an aid to histopathological diagnosis of breast cancer, in particular in the discrimination between benign and malignant tumours.

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Published in: PLOS ONE
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Qatar National Research Fund (NPRP 7 - 1267 - 3 - 328), Development of infrared and Raman imaging for objective scoring of breast cancer tissue sections.



  • English


Public Library of Science (PLoS)

Publication Year

  • 2019

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Institution affiliated with

  • Hamad Bin Khalifa University
  • Qatar Biomedical Research Institute - HBKU
  • Qatar University
  • College of Arts and Sciences - QU
  • Al Ahli Hospital