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Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations

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submitted on 2024-04-04, 09:51 and posted on 2024-04-04, 09:51 authored by Noor A. Al-Maslamani, Rachel Oldershaw, Simon Tew, Jude Curran, Pieter D’Hooghe, Kazuhiro Yamamoto, Henning F. Horn

Autologous chondrocyte implantation (ACI) is a cell therapy to repair cartilage defects. In ACI a biopsy is taken from a non-load bearing area of the knee and expanded in-vitro. The expansion process provides the benefit of generating a large number of cells required for implantation; however, during the expansion these cells de-differentiate and lose their chondrocyte phenotype. In this review we focus on examining the de-differentiation phenotype from a mechanobiology and biophysical perspective, highlighting some of the nuclear mechanics and chromatin changes in chondrocytes seen during the expansion process and how this relates to the gene expression profile. We propose that manipulating chondrocyte nuclear architecture and chromatin organization will highlight mechanisms that will help to preserve the chondrocyte phenotype.

Other Information

Published in: Cells
License: https://creativecommons.org/licenses/by/4.0/
See article on publisher's website: https://dx.doi.org/10.3390/cells11244011

History

Language

  • English

Publisher

MDPI

Publication Year

  • 2022

License statement

This Item is licensed under the Creative Commons Attribution 4.0 International License.

Institution affiliated with

  • Aspire Zone Foundation
  • Aspetar Orthopaedic and Sports Medicine Hospital - AZF
  • Hamad Bin Khalifa University
  • College of Health and Life Sciences - HBKU